Persistent infection of SARS coronavirus in colonic cells in vitro
Identifieur interne : 005A94 ( Main/Exploration ); précédent : 005A93; suivant : 005A95Persistent infection of SARS coronavirus in colonic cells in vitro
Auteurs : Paul K. S. Chan [Hong Kong] ; Ka-Fai To [Hong Kong] ; Anthony W. I. Lo [Hong Kong] ; Jo L. K. Cheung [Hong Kong] ; IDA CHU [Hong Kong] ; Florence W. L. Au [Hong Kong] ; Joanna H. M. Tong [Hong Kong] ; John S. Tam [Hong Kong] ; Joseph J. Y. Sung [Hong Kong] ; Ho-Keung Ng [Hong Kong]Source :
- Journal of medical virology [ 0146-6615 ] ; 2004.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Antigènes viraux (analyse), Antigènes viraux (immunologie), Carboxypeptidases (biosynthèse), Carboxypeptidases (métabolisme), Cytoplasme (), Cytoplasme (virologie), Côlon (cytologie), Côlon (virologie), Effet cytopathogène viral, Humains, Hybridation in situ, Lignée cellulaire tumorale, Microscopie électronique, Peptidyl-Dipeptidase A, RT-PCR, Récepteurs viraux, Technique d'immunofluorescence indirecte, Tumeurs colorectales, Virus du SRAS (croissance et développement), Virus du SRAS (immunologie).
- MESH :
- analyse : ARN viral, Antigènes viraux.
- biosynthèse : Carboxypeptidases.
- croissance et développement : Virus du SRAS.
- cytologie : Côlon.
- immunologie : Antigènes viraux, Virus du SRAS.
- métabolisme : Carboxypeptidases.
- virologie : Cytoplasme, Côlon.
- Pascal (Inist)
- Coronavirus, Cytoplasme, Effet cytopathogène viral, Humains, Hybridation in situ, Infection persistante, In vitro, Côlon, Lignée cellulaire tumorale, Microscopie électronique, Peptidyl-Dipeptidase A, RT-PCR, Récepteurs viraux, Syndrome respiratoire aigu sévère, Technique d'immunofluorescence indirecte, Tumeurs colorectales, Virus syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Antigens, Viral (analysis), Antigens, Viral (immunology), Carboxypeptidases (biosynthesis), Carboxypeptidases (metabolism), Cell Line, Tumor, Colon, Colon (cytology), Colon (virology), Colorectal Neoplasms, Coronavirus, Cytopathogenic Effect, Viral, Cytoplasm (chemistry), Cytoplasm (virology), Fluorescent Antibody Technique, Indirect, Humans, In Situ Hybridization, In vitro, Microscopy, Electron, Peptidyl-Dipeptidase A, Persistent infection, RNA, Viral (analysis), Receptors, Virus, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (growth & development), SARS Virus (immunology), Severe acute respiratory syndrome, Severe acute respiratory syndrome virus.
- MESH :
- chemical , analysis : Antigens, Viral, RNA, Viral.
- chemical , biosynthesis : Carboxypeptidases.
- chemical , immunology : Antigens, Viral.
- chemical , metabolism : Carboxypeptidases.
- chemistry : Cytoplasm.
- cytology : Colon.
- growth & development : SARS Virus.
- immunology : SARS Virus.
- virology : Colon, Cytoplasm.
- Cell Line, Tumor, Colorectal Neoplasms, Cytopathogenic Effect, Viral, Fluorescent Antibody Technique, Indirect, Humans, In Situ Hybridization, Microscopy, Electron, Peptidyl-Dipeptidase A, Receptors, Virus, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) can produce gastrointestinal symptoms. The intestinal tract is the only extrapulmonary site where viable viruses have been detected. This study examined seven established human intestinal cell lines, DLD-1, HCT-116, HT-29, LoVo, LS-180, SW-480 and SW-620, for their permissiveness to SARS-CoV infection. The results showed that only LoVo cells were permissive to SARS-CoV infection as evident by positive findings from indirect immunofluorescence staining for intracellular viral antigens, in situ hybridization for intracellular viral RNA, and electron microscopy for intracellular viral particles. In contrast to Vero cells, SARS-CoV did not produce cytopathic effects on LoVo cells. However, LoVo cells were found to be highly permissive for productive infection with a high viral titre (>3×107 viral copies/ml) produced in culture supernatant following a few days of incubation. SARS-CoV established a stable persistent chronic infection that could be maintained after multiple passages. Being a cell line of human origin, LoVo cells could be a useful in vitro model for studying the biology and persistent infection of SARS-CoV. Our results on the expression of angiotensin-converting enzyme 2 (ACE2), a recently identified cellular receptor for SARS-CoV, in these cell lines indicated that it might not be the sole determinant for cells to be susceptible to SARS-CoV infection.
Affiliations:
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Le document en format XML
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Tong</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S." last="Tam">John S. Tam</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J. 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Sung</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Ng, Ho Keung" sort="Ng, Ho Keung" uniqKey="Ng H" first="Ho-Keung" last="Ng">Ho-Keung Ng</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0487364</idno><date when="2004">2004</date><idno type="stanalyst">PASCAL 04-0487364 INIST</idno><idno type="RBID">Pascal:04-0487364</idno><idno type="wicri:Area/PascalFrancis/Corpus">000813</idno><idno type="wicri:Area/PascalFrancis/Curation">000177</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000801</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000801</idno><idno type="wicri:doubleKey">0146-6615:2004:Chan P:persistent:infection:of</idno><idno type="wicri:Area/Main/Merge">005F52</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:15258961</idno><idno type="wicri:Area/PubMed/Corpus">002C58</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002C58</idno><idno type="wicri:Area/PubMed/Curation">002C58</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002C58</idno><idno type="wicri:Area/PubMed/Checkpoint">002B60</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002B60</idno><idno type="wicri:Area/Ncbi/Merge">000954</idno><idno type="wicri:Area/Ncbi/Curation">000954</idno><idno type="wicri:Area/Ncbi/Checkpoint">000954</idno><idno type="wicri:doubleKey">0146-6615:2004:Chan P:persistent:infection:of</idno><idno type="wicri:Area/Main/Merge">005603</idno><idno type="wicri:Area/Main/Curation">005A94</idno><idno type="wicri:Area/Main/Exploration">005A94</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Persistent infection of SARS coronavirus in colonic cells in vitro</title><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="To, Ka Fai" sort="To, Ka Fai" uniqKey="To K" first="Ka-Fai" last="To">Ka-Fai To</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Lo, Anthony W I" sort="Lo, Anthony W I" uniqKey="Lo A" first="Anthony W. I." last="Lo">Anthony W. I. Lo</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L. K." last="Cheung">Jo L. K. Cheung</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Ida Chu" sort="Ida Chu" uniqKey="Ida Chu" last="Ida Chu">IDA CHU</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Au, Florence W L" sort="Au, Florence W L" uniqKey="Au F" first="Florence W. L." last="Au">Florence W. L. Au</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Tong, Joanna H M" sort="Tong, Joanna H M" uniqKey="Tong J" first="Joanna H. M." last="Tong">Joanna H. M. Tong</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S." last="Tam">John S. Tam</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J. Y." last="Sung">Joseph J. Y. Sung</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Ng, Ho Keung" sort="Ng, Ho Keung" uniqKey="Ng H" first="Ho-Keung" last="Ng">Ho-Keung Ng</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital</s1><s2>Shatin, New Territories</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Shatin, New Territories</wicri:noRegion><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of medical virology</title><title level="j" type="abbreviated">J. med. virol.</title><idno type="ISSN">0146-6615</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of medical virology</title><title level="j" type="abbreviated">J. med. virol.</title><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, Viral (analysis)</term><term>Antigens, Viral (immunology)</term><term>Carboxypeptidases (biosynthesis)</term><term>Carboxypeptidases (metabolism)</term><term>Cell Line, Tumor</term><term>Colon</term><term>Colon (cytology)</term><term>Colon (virology)</term><term>Colorectal Neoplasms</term><term>Coronavirus</term><term>Cytopathogenic Effect, Viral</term><term>Cytoplasm (chemistry)</term><term>Cytoplasm (virology)</term><term>Fluorescent Antibody Technique, Indirect</term><term>Humans</term><term>In Situ Hybridization</term><term>In vitro</term><term>Microscopy, Electron</term><term>Peptidyl-Dipeptidase A</term><term>Persistent infection</term><term>RNA, Viral (analysis)</term><term>Receptors, Virus</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (growth & development)</term><term>SARS Virus (immunology)</term><term>Severe acute respiratory syndrome</term><term>Severe acute respiratory syndrome virus</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Antigènes viraux (analyse)</term><term>Antigènes viraux (immunologie)</term><term>Carboxypeptidases (biosynthèse)</term><term>Carboxypeptidases (métabolisme)</term><term>Cytoplasme ()</term><term>Cytoplasme (virologie)</term><term>Côlon (cytologie)</term><term>Côlon (virologie)</term><term>Effet cytopathogène viral</term><term>Humains</term><term>Hybridation in situ</term><term>Lignée cellulaire tumorale</term><term>Microscopie électronique</term><term>Peptidyl-Dipeptidase A</term><term>RT-PCR</term><term>Récepteurs viraux</term><term>Technique d'immunofluorescence indirecte</term><term>Tumeurs colorectales</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Carboxypeptidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carboxypeptidases</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term><term>Antigènes viraux</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Carboxypeptidases</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cytoplasm</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Côlon</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Colon</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Carboxypeptidases</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cytoplasme</term><term>Côlon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Colon</term><term>Cytoplasm</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Colorectal Neoplasms</term><term>Cytopathogenic Effect, Viral</term><term>Fluorescent Antibody Technique, Indirect</term><term>Humans</term><term>In Situ Hybridization</term><term>Microscopy, Electron</term><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Cytoplasme</term><term>Effet cytopathogène viral</term><term>Humains</term><term>Hybridation in situ</term><term>Infection persistante</term><term>In vitro</term><term>Côlon</term><term>Lignée cellulaire tumorale</term><term>Microscopie électronique</term><term>Peptidyl-Dipeptidase A</term><term>RT-PCR</term><term>Récepteurs viraux</term><term>Syndrome respiratoire aigu sévère</term><term>Technique d'immunofluorescence indirecte</term><term>Tumeurs colorectales</term><term>Virus syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) can produce gastrointestinal symptoms. The intestinal tract is the only extrapulmonary site where viable viruses have been detected. This study examined seven established human intestinal cell lines, DLD-1, HCT-116, HT-29, LoVo, LS-180, SW-480 and SW-620, for their permissiveness to SARS-CoV infection. The results showed that only LoVo cells were permissive to SARS-CoV infection as evident by positive findings from indirect immunofluorescence staining for intracellular viral antigens, in situ hybridization for intracellular viral RNA, and electron microscopy for intracellular viral particles. In contrast to Vero cells, SARS-CoV did not produce cytopathic effects on LoVo cells. However, LoVo cells were found to be highly permissive for productive infection with a high viral titre (>3×10<sup>7</sup> viral copies/ml) produced in culture supernatant following a few days of incubation. SARS-CoV established a stable persistent chronic infection that could be maintained after multiple passages. Being a cell line of human origin, LoVo cells could be a useful in vitro model for studying the biology and persistent infection of SARS-CoV. Our results on the expression of angiotensin-converting enzyme 2 (ACE2), a recently identified cellular receptor for SARS-CoV, in these cell lines indicated that it might not be the sole determinant for cells to be susceptible to SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>Hong Kong</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><country name="Hong Kong"><noRegion><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name></noRegion><name sortKey="Au, Florence W L" sort="Au, Florence W L" uniqKey="Au F" first="Florence W. L." last="Au">Florence W. L. Au</name><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K. S." last="Chan">Paul K. S. Chan</name><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L. K." last="Cheung">Jo L. K. Cheung</name><name sortKey="Ida Chu" sort="Ida Chu" uniqKey="Ida Chu" last="Ida Chu">IDA CHU</name><name sortKey="Lo, Anthony W I" sort="Lo, Anthony W I" uniqKey="Lo A" first="Anthony W. I." last="Lo">Anthony W. I. Lo</name><name sortKey="Ng, Ho Keung" sort="Ng, Ho Keung" uniqKey="Ng H" first="Ho-Keung" last="Ng">Ho-Keung Ng</name><name sortKey="Sung, Joseph J Y" sort="Sung, Joseph J Y" uniqKey="Sung J" first="Joseph J. Y." last="Sung">Joseph J. Y. Sung</name><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S." last="Tam">John S. Tam</name><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S." last="Tam">John S. Tam</name><name sortKey="To, Ka Fai" sort="To, Ka Fai" uniqKey="To K" first="Ka-Fai" last="To">Ka-Fai To</name><name sortKey="Tong, Joanna H M" sort="Tong, Joanna H M" uniqKey="Tong J" first="Joanna H. M." last="Tong">Joanna H. M. Tong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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